project - EIP-AGRI Operational Group

INNOLEG - Innovative systems for obtaining LegHemoglobin for meat analogue products
INNOLEG- Sistemas innovadores para la obtención de la LegHemoglobina para productos análogos de la carne

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Objectives

The project presented here aims to develop a process for obtaining LegHemoglobin in Saccharomyces cerevisiae yeast, as obtaining its original source - infected nodules of soybean root - is not feasible in large quantities. It also aims to study the industrial viability of this process, and to evaluate the use of protein for improving plant preparations intended for human consumption.

Objectives

El proyecto que se presenta tiene como objetivo desarrollar un proceso para la obtención de LegHemoglobina en la levadura Saccharomyces cerevisiae, ya que la obtención masiva de su fuente original, los nódulos infectados de la raíz de la soja, no es viable. También se pretende estudiar la viabilidad industrial de este proceso y avaluar el uso de proteínas para la mejora de las preparaciones vegetales destinadas a la alimentación humana.
Descripción de las actuaciones previstas en el proyecto

Activities

• 1. Redesign and synthesise the sequence to be expressed to optimise its production.
• 2. Develop vectors that enable its expression and secretion in the culture medium.
•  3. Identify and eliminate bottlenecks in production on the laboratory scale, redesigning strains if necessary.
• 4. Carry out production tests in 5 L scale bioreactors in a pilot plant, and design protein purification processes.
• 5. Carry out production tests in 50 L scale bioreactors in a pilot plant, and design protein purification processes.
• 6. Perform protein addition assays in the end products, and evaluation of the characteristics conferred.
• 7. Dissemination of the project

Activities

• 1. Rediseño y síntesis de la secuencia a expresar para optimizar su producción.
• 2. Desarrollo de vectores que permitan su expresión y secreción en el medio de cultivo.
• 3. Detección y eliminación de cuellos de botella productivos a escala de laboratorio, rediseñando las cepas si es necesario.
• 4. Pruebas de producción en biorreactores a escala de 5 L en planta piloto y diseño de procesos de purificación de proteínas.

• 5. Pruebas de producción en biorreactores a escala de 50 L en planta piloto y diseño de procesos de purificación de proteínas.
• 6. Ensayos de adición de proteínas a productos finales y evaluación de características conferidas.
• 7. Difusión del proyecto

Kontext

There is currently a market shortage of vegetable protein-based products with the characteristics of as many types of meat as possible. These products are known as meat analogues, and their main characteristic is that the consumer can use them as a substitute for the meat products that they are consuming.
The most obvious example is a beef burger. A meat analogue for this product would be a dish based on vegetable protein, such as soy, which would be cooked just like a beef burger and have the same appearance, flavour and colour.
The texture of this type of product is now quite effective, as many analogues have a consistency that is very similar to meat products. However, what is lacking is a solution that provides meat's typical flavour and colour, especially while the products are cooking.
LegHemoglobin, a vegetable protein derived from soybean nodules, provides the colour and basic flavour of meat for use in vegetable by-products, and in mixtures of meat and vegetables. This protein is obtained by modifying a yeast that secretes it, and it is subsequently purified for use in industry as an ingredient for vegetarian applications.
No company in Europe is currently able to produce this ingredient, and this project aims to develop a method to obtain it. Being pioneers in its development would enhance the competitiveness of Catalan companies.

Project details
Main funding source
Rural development 2014-2020 for Operational Groups
Rural Development Programme
2014ES06RDRP009 Spain - Rural Development Programme (Regional) - Cataluña
Ort
Main geographical location
Girona

€ 212000

Total budget

Total contributions from EAFRD, national co-financing, additional national financing and other financing.

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Contacts

Project coordinator