project - Research and innovation
Porcine immune reactions towards bacterial resistence (Actinobacillus pleuropneumoniae)
Porcine immune reactions towards bacterial resistence (Actinobacillus pleuropneumoniae)
Objectives
The porcine respiratory tract pathogen Actinobacillus pleuropneumonie (A.pp.). leads to high economic losses to the swine industry wordwide. This bacterium ist the agent of the contagious porcine pleuropneumonia causing peracute, acute, chronic or subclinical courses of disease.
Objectives
The porcine respiratory tract pathogen Actinobacillus pleuropneumonie (A.pp.). leads to high economic losses to the swine industry wordwide. This bacterium ist the agent of the contagious porcine pleuropneumonia causing peracute, acute, chronic or subclinical courses of disease.
Activities
Several studies focused onto the expression of virulence factors of A.pp. during experimental infections in swine. Changes in the composition of bronchoalveolar lavage fluid during the course of disease due to inflammation have been examined by 2-D-gel-electrophoresis and other analytical methods. In addition, transcriptional analyses supported transcriptional changes in host tissue as well as in A.pp. at different stages of infections and growth conditions.
Porcine immune reactions during the persistence of A.pp. serotype 2 will be analyzed. Acute early immune reactions a few hours after infection will be compared to those at later stages of infection
The hypothesis is, that differences in courses of disease, in the extent of pathological lung alterations and the reisolation rate will occur between different serotypes of A.pp.. These differences willl be reflected by changes in the composition of bronchoalveolar lavage fluid.
The proteom and immunoproteom of the A.pp. strains as well as onto the FTIR spectroscopy analyses will be analyzed to study metabolic changes of the bacteria isolated from different anatomical sites of persistence and at different stages of infection.
Activities
Several studies focused onto the expression of virulence factors of A.pp. during experimental infections in swine. Changes in the composition of bronchoalveolar lavage fluid during the course of disease due to inflammation have been examined by 2-D-gel-electrophoresis and other analytical methods. In addition, transcriptional analyses supported transcriptional changes in host tissue as well as in A.pp. at different stages of infections and growth conditions.
Porcine immune reactions during the persistence of A.pp. serotype 2 will be analyzed. Acute early immune reactions a few hours after infection will be compared to those at later stages of infection
The hypothesis is, that differences in courses of disease, in the extent of pathological lung alterations and the reisolation rate will occur between different serotypes of A.pp.. These differences willl be reflected by changes in the composition of bronchoalveolar lavage fluid.
The proteom and immunoproteom of the A.pp. strains as well as onto the FTIR spectroscopy analyses will be analyzed to study metabolic changes of the bacteria isolated from different anatomical sites of persistence and at different stages of infection.
Project details
- Main funding source
- Other public (national, regional) research funds
- Agricultural sectors
- Pigs
Project keyword
Contacts
Project coordinator
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Project coordinator
Project partners
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University of Veterinary Medicine, Vienna, Austria: Clinic for swine
Project partner
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University of Veterinary Medicine, Vienna, Austria: Institute of Microbiology
Project partner
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University of Veterinary Medicine, Vienna, Austria: Institute of Immunology
Project partner
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University of Veterinary Medicine, Hannover, Germany: Clinic for swine and small ruminants
Project partner
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University of Veterinary Medicine, Hannover, Germany: Institute of Microbiology
Project partner
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National Veterinary Institute,Technical University of Denmark: Innate Immunology Group
Project partner