The anticipated results of the project: mitigation of greenhouse gases at feed production, storage and usage in the ruminant digestive tract and also from manure in barns and storage places by application of the latest achievements in biotechnology and with no negative effects on animal production, its quality and cost price. It is expected that the measures applied within the Project will allow to reduce the amount of gas emission to the environment, to improve the quality of feeds, to lower nutrient losses in feeds, and to improve nutrient assimilation and productivity of lactating cows. The public and surplus value of the Project is based on the possibility to spread information in rural areas about greenhouse gas sources in agriculture and the measures that allow to mitigate the intensity of gas emissions at feed production, animal feeding and by improving farming practice This can be seen at all stages of silage production, beginning in the field   through plant cell breathing to silage fermentation and during secondary fermentation  at feeding. Higher nutrient digestibility and assimilation reduces the amount of excrements and other by-products of digestion (gases) getting into the environment. Normalization of these processes in the cow body by developing optimum food allowances, order of feeding and effective methods of feed preparation will secure the highest feed conversion and at the same time reduce the amount of gases emitted at nutrient fermentation. Thus, the usage of biotechnology products at ensilage and feeding will not reduce cow productivity but will mitigate the emission of greenhouse gases both from animals and from feed preparation.